Reservoirs of Staphylococcus spp. and Streptococcus spp. Associated with Intramammary Infections of Dairy Cows
- To design cost-effective prevention strategies against mastitis in dairy cow farms, knowledge about infection pathways of causative pathogens is necessary. Therefore, we investigated the reservoirs of bacterial strains causing intramammary infections in one dairy cow herd. Quarter foremilk samples (n = 8056) and milking- and housing-related samples (n = 251; from drinking troughs, bedding material, walking areas, cow brushes, fly traps, milking liners, and milker gloves), were collected and examined using culture-based methods. Species were identified with MALDI-TOF MS, and selected Staphylococcus and Streptococcus spp. typed with randomly amplified polymorphic DNA-PCR. Staphylococci were isolated from all and streptococci from most investigated locations. However, only for Staphylococcus aureus, matching strain types (n = 2) were isolated from milk and milking-related samples (milking liners and milker gloves). Staphylococcus epidermidis and Staphylococcus haemolyticus showed a large genetic diversity without any matches of strain types from milk and other samples. Streptococcus uberis was the only Streptococcus spp. isolated from milk and milking- or housing-related samples. However, no matching strains were found. This study underlines the importance of measures preventing the spread of Staphylococcus aureus between quarters during milking.
Author: | Svenja WoudstraORCiD, Nicole WenteORCiD, Yanchao ZhangORCiD, Stefanie LeimbachORCiD, Carsten Kirkeby, Maya Katrin Gussmann, Volker KrömkerORCiD |
---|---|
URN: | urn:nbn:de:bsz:960-opus4-28759 |
DOI: | https://doi.org/10.25968/opus-2875 |
DOI original: | https://doi.org/10.3390/pathogens12050699 |
ISSN: | 2076-0817 |
Parent Title (English): | Pathogens |
Document Type: | Article |
Language: | English |
Year of Completion: | 2023 |
Publishing Institution: | Hochschule Hannover |
Release Date: | 2023/06/06 |
Tag: | environmental reservoirs; mastitis prevention; pathogen reservoirs; staphylococci; streptococci; transmission pathways |
GND Keyword: | Euterentzündung; Prävention; Staphylococcus; Streptococcus; Pathogener Mikroorganismus; Krankheitsübertragung |
Volume: | 12 |
Issue: | 5 |
Article Number: | 699 |
Note: | Gefördert mit Mitteln des Publikationsfonds der Bibliothek der Hochschule Hannover. |
Link to catalogue: | 1858413664 |
Institutes: | Fakultät II - Maschinenbau und Bioverfahrenstechnik |
DDC classes: | 630 Landwirtschaft, Veterinärmedizin |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |