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Evaluation of DNA repair capacity in parents of pediatric patients diagnosed with autism spectrum disorder using the comet assay procedure

  • Background: Autism Spectrum Disorder (ASD) is characterized by impairments in social communication, limited repetitive behaviors, impaired language development, and interest or activity patterns, which include a group complex neurodevelopmental syndrome with diverse phenotypes that reveal considerable etiological and clinical heterogeneity and are also considered one of the most heritable disorders (over 90%). Genetic, epigenetic, and environmental factors play a role in the development of ASD. Aim: This study was designed to investigate the extent of DNA damage in parents of autistic children by treating peripheral blood mononuclear cells (PBMCs) with bleomycin and hydrogen peroxide (H2O2). Methods: Peripheral blood mononuclear cells (PBMCs) were isolated by the Ficoll method and treated with a specific concentration of bleomycin and H2O2 for 30 min and 5 min, respectively. Then, the degree of DNA damage was analyzed by the alkaline comet assay or single cell gel electrophoresis (SCGE), an effective way to measure DNA fragmentation in eukaryotic cells. Results: Our findings revealed that there is a significant difference in the increase of DNA damage in parents with affected children compared to the control group, which can indicate the inability of the DNA molecule repair system. Furthermore, our study showed a significant association between fathers’ occupational difficulties (exposed to the influence of environmental factors), as well as family marriage, and suffering from ASD in offspring. Conclusion: Our results suggested that the influence of environmental factors on parents of autistic children may affect the development of autistic disorder in their offspring. Subsequently, based on our results, investigating the effect of environmental factors on the amount of DNA damage in parents with affected children requires more studies.

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Metadaten
Author:Mansoureh Akouchekian, Rasoul Alizadeh, Fatemeh Beiranvandi, Masoumeh Dehghan Manshadi, Fatemeh TaherizadehORCiDGND, Mitra Hakim Shooshtari
URN:urn:nbn:de:bsz:960-opus4-30105
DOI:https://doi.org/10.25968/opus-3010
DOI original:https://doi.org/10.1016/j.ibneur.2023.10.003
ISSN:2667-2421
Parent Title (English):IBRO Neuroscience Reports
Document Type:Article
Language:English
Year of Completion:2023
Publishing Institution:Hochschule Hannover
Release Date:2023/11/16
Tag:ASD; Autism; Autism spectrum disorder; DNA damage; DNA repair
GND Keyword:Autismus; DNS-Reparatur; DNS-Schädigung; Comet Assay
Volume:15
First Page:304
Last Page:309
Link to catalogue:1885952074
Institutes:Fakultät III - Medien, Information und Design
DDC classes:610 Medizin, Gesundheit
Licence (German):License LogoCreative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International