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Intermediate Cluster Disinfection: Which Disinfection Solution Is Most Effective on Milking Liners? A Comparison of Microorganism Reduction on Liner Inner Surfaces Using Quantitative Swab Sampling Technique

  • During machine milking, pathogenic microorganisms can be transmitted from cow to cow through liners. Therefore, in Germany, a spray method for the intermediate disinfection of the milking cluster is often used for prevention. This method of cluster disinfection is easy to perform, requires little time and no extra materials, and the disinfection solution is safe from outside contamination in the spray bottle. Since no data on a systematic efficacy trial are available, the aim of this study was to determine the microbial reduction effect of intermediate disinfection. Therefore, laboratory and field trials were conducted. In both trials, two sprays of 0.85 mL per burst of different disinfectant solutions were sprayed into the contaminated liners. For sampling, a quantitative swabbing method using a modified wet–dry swab (WDS) technique based on DIN 10113-1: 1997-07 was applied. Thus, the effectiveness of disinfectants based on Peracetic Acid, Hydrogen Peroxide and Plasma-Activated Buffered Solution (PABS) was compared. In the laboratory trial, the inner surfaces of liners were contaminated with pure cultures of Escherichia (E.) coli, Staphylococcus (S.) aureus, Streptococcus (Sc.) uberis and Sc. agalactiae. The disinfection of the contaminated liners with the disinfectants resulted in a significant reduction in bacteria with values averaging 1 log for E. coli, 0.7 log for S. aureus, 0.7 log for Sc. uberis and 0.8 log for Sc. agalactiae. The highest reduction was obtained for contamination with E. coli (1.3 log) and Sc. uberis (0.8 log) when PABS was applied and for contamination with S. aureus (1.1 log) and Sc. agalactiae (1 log) when Peracetic Acid Solution (PAS) was used. Treatment with sterile water only led to an average reduction of 0.4 log. In the field trial, after the milking of 575 cows, the liners were disinfected and the total microorganism count from the liner surface was performed. The reduction was measured against an untreated liner within the cluster. Although a reduction in microorganisms was achieved in the field trial, it was not significant. When using PAS, a log reduction of 0.3 was achieved; when using PABS, a log reduction of 0.2 was obtained. The difference between the two disinfection methods was also not significant. Treatment with sterile water only led to a reduction of 0.1 log. The results show that spray disinfection under these circumstances does result in a reduction in the bacteria on the milking liner surface, but for effective disinfection a higher reduction would be preferred.

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Metadaten
Author:Sabrina ScheibORCiD, Stefanie LeimbachORCiD, Georg AvramidisORCiD, Martin Bellmann, Julia Nitz, Christian Ochs, Anne Tellen, Nicole WenteORCiD, Yanchao ZhangORCiD, Wolfgang ViölORCiD, Volker KrömkerORCiD
URN:urn:nbn:de:bsz:960-opus4-25664
DOI:https://doi.org/10.25968/opus-2566
DOI original:https://doi.org/10.3390/pathogens12040560
ISSN:2076-0817
Parent Title (English):Pathogens
Document Type:Article
Language:English
Year of Completion:2023
Publishing Institution:Hochschule Hannover
Release Date:2023/04/11
Tag:Peracetic Acid Solution; Plasma-Activated Buffered Solution; mastitis prevention; surface microbial count; wet–dry swab technique
GND Keyword:Maschinenmelken; Krankheitsübertragung; Desinfektion; Effizienzanalyse
Volume:12
Issue:4
Article Number:560
Page Number:14
Note:
Gefördert mit Mitteln des Publikationsfonds der Bibliothek der Hochschule Hannover.
Link to catalogue:1850603197
Institutes:Fakultät II - Maschinenbau und Bioverfahrenstechnik
DDC classes:630 Landwirtschaft, Veterinärmedizin
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International