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Piper crocatum Ruiz & Pav. ameliorates wound healing through p53,E-cadherin and SOD1 pathways on wounded hyperglycemia fibroblasts

  • Introduction: Piper crocatum Ruiz & Pav (P. crocatum) has been reported to accelerate the diabetic wound healing process empirically. Some studies showed the benefits of P. crocatum in treating various diseases but its mechanisms in diabetic wound healing have never been reported. In the present study we investigated the diabetic wound healing activity of the active fraction of P. crocatum on wounded hyperglycemia fibroblasts (wHFs). Methods: Bioassay-guided fractionation was performed to get the most active fraction. The selected active fraction was applied to wHFs within 72 h incubation. Mimicking a diabetic condition was done using basal glucose media containing an additional 17 mMol/L D-glucose. A wound was simulated via the scratch assay. The collagen deposition was measured using Picro-Sirius Red and wound closure was measured using scratch wound assay. Underlying mechanisms through p53, aSMA, SOD1 and Ecadherin were measured using western blotting. Results: We reported that FIV is the most active fraction of P. crocatum. We confirmed that FIV\(7.81 mg/ml, 15.62 mg/ml, 31.25 mg/ml, 62.5 mg/ml, and 125 mg/ml) induced the collagen deposition and wound closure of wHFs. Furthermore, FIV treatment (7.81 mg/ml, 15.62 mg/ml, 31.25 mg/ml) down-regulated the protein expression level of p53 and up-regulated the protein expression levels of aSMA, E-cadherin, and SOD1. Discussion/conclusions: Our findings suggest that ameliorating collagen deposition and wound closure through protein regulation of p53, aSMA, E-cadherin, and SOD1 are some of the mechanisms by which FIV of P. crocatum is involved in diabetic wound healing therapy.

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Author:Andina Setyawati, Mae Sri Hartati Wahyuningsih, Dwi Aris Agung Nugrahaningsih, Christantie Effendy, Firas Fneish, Gerhard FortwengelORCiDGND
DOI original:https://doi.org/https://doi.org/10.1016/j.sjbs.2021.08.039
Parent Title (English):Saudi Journal of Biological Sciences
Document Type:Article
Year of Completion:2021
Publishing Institution:Hochschule Hannover
Release Date:2021/12/02
Tag:Diabetic wound healing; E-cadherin; Mechanism; Piper crocatum Ruiz & Pav; SOD1; p53
GND Keyword:Diabetes mellitus; Wundheilung; Fraktionierung
First Page:7257
Last Page:7268
Link to catalogue:1796766763
Institutes:Fakult├Ąt III - Medien, Information und Design
DDC classes:610 Medizin, Gesundheit
Licence (German):License LogoCreative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International