Volltext-Downloads (blau) und Frontdoor-Views (grau)
(Leider keine statistischen Daten vom 26.05. – 18.06.2018)
  • search hit 1 of 1
Back to Result List

Recommendation for a standardised method of broth microdilution susceptibility testing for porcine Bordetella bronchiseptica

  • The objective was to establish and standardise a broth microdilution susceptibility testing method for porcine Bordetella (B.) bronchiseptica. B. bronchiseptica isolates from different geographical regions and farms were genotyped by macrorestriction analysis and subsequent pulsed-field gel electrophoresis. One reference and one type strain plus two field isolates of B. bronchiseptica were chosen to analyse growth curves in four different media: cation-adjusted Mueller-Hinton broth (CAMHB) with and without 2% lysed horse blood, Brain-Heart-Infusion (BHI), and Caso broth. The growth rate of each test strain in each medium was determined by culture enumeration and the suitability of CAMHB was confirmed by comparative statistical analysis. Thereafter, reference and type strain and eight epidemiologically unrelated field isolates of B. bronchiseptica were used to test the suitability of a broth microdilution susceptibility testing method following CLSI-approved performance standards given in document VET01-A4. Susceptibility tests, using 20 antimicrobial agents, were performed in five replicates, and data were collected after 20 and 24 hours incubation and statistically analysed. Due to the low growth rate of B. bronchiseptica, an incubation time of 24 hours resulted in significantly more homogeneous minimum inhibitory concentrations after five replications compared to a 20-hour incubation. An interlaboratory comparison trial including susceptibility testing of 24 antimicrobial agents revealed a high mean level of reproducibility (97.9%) of the modified method. Hence, in a harmonization for broth microdilution susceptibility testing of B. bronchiseptica, an incubation time of 24 hours in CAMHB medium with an incubation temperature of 35°C and an inoculum concentration of approximately 5 x 105 cfu/ml was proposed.

Download full text files

Export metadata

Additional Services

Share in Twitter Search Google Scholar

Statistics

frontdoor_oas
Metadaten
Author:Prüller Sandra, Cornelia FrömkeGND, Heike Kaspar, Günter Klein, Lothar Kreienbrock, Corinna Kehrenberg
URN:urn:nbn:de:bsz:960-opus4-11186
DOI:https://doi.org/10.1371/journal.pone.0123883
Parent Title (English):PLoS ONE
Document Type:Article
Language:English
Year of Completion:2015
Release Date:2017/07/24
Tag:Bortedella; Germany; antibiotics; antimicrobials; bacterial pathogens; blood; broth microdilution; swine
Volume:2015
Issue:10(4)
Institutes:Fakultät III - Medien, Information und Design
DDC classes:630 Landwirtschaft, Veterinärmedizin
Licence (German):License LogoCreative Commons - Namensnennung 4.0