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Biofilm Challenge: Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci

  • Mastitis poses a considerable threat to productivity and to animal welfare on modern dairy farms. However, the common way of antibiotic treatment does not always lead to a cure. Unsuccessful cures can, among other reasons, occur due to biofilm formation of the causative agent. This has attracted interest from researchers to introduce promising alternative therapeutic approaches, such as the use of beneficial lactic acid bacteria (LAB). In fact, using LAB for treating mastitis probably requires the formation of a beneficial biofilm by the probiotic bacteria. The present study investigated the ability of five LAB strains, selected on the basis of results from previous studies, to remove and to replace pathogenic biofilms in vitro. For this purpose, Staphylococcus (S.) aureus ATCC 12,600 and two strains—S. xylosus (35/07) and S. epidermidis (575/08)—belonging to the group of coagulase negative staphylococci (CNS) were allowed to form biofilms in a 96-well plate. Subsequently, the LAB were added to the well. The biofilm challenge was evaluated by scraping off and suspending the biofilm cells, followed by a plate count of serial dilutions using selective media. All the LAB strains successfully removed the staphylococcal biofilms. However, only Lactobacillus (L.) rhamnosus ATCC 7469 and L. plantarum 2/37 formed biofilms of their own to replace the pathogenic ones.

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Metadaten
Author:Jonathan K. Wallis, Volker KrömkerORCiDGND, Jan-Hendrik PaduchGND
URN:urn:nbn:de:bsz:960-opus4-13526
DOI original:https://doi.org/10.3390/foods8020079
ISSN:2304-8158
Parent Title (English):Foods
Document Type:Article
Language:English
Year of Completion:2019
Publishing Institution:Hochschule Hannover
Release Date:2019/06/24
Tag:biofilm; lactic acid bacteria; mastitis; probiotic potential; staphylococci
GND Keyword:Biofilm; Brustdrüsenentzündung; Milchsäurebakterien; Staphylococcaceae
Volume:2019
Issue:8(2)
Institutes:Fakultät II - Maschinenbau und Bioverfahrenstechnik
DDC classes:630 Landwirtschaft, Veterinärmedizin
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International