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Measured Effects of Wnt3a on Proliferation of HEK293T Cells Depend on the Applied Assay

  • The Wnt signaling pathway has been associated with many essential cell processes. This study aims to examine the effects of Wnt signaling on proliferation of cultured HEK293T cells. Cells were incubated with Wnt3a, and the activation of the Wnt pathway was followed by analysis of the level of the β-catenin protein and of the expression levels of the target genes MYC and CCND1. The level of β-catenin protein increased up to fourfold. While the mRNA levels of c-Myc and cyclin D1 increased slightly, the protein levels increased up to a factor of 1.5. Remarkably, MTT and BrdU assays showed different results when measuring the proliferation rate of Wnt3a stimulated HEK293T cells. In the BrdU assays an increase of the proliferation rate could be detected, which correlated to the applied Wnt3a concentration. Oppositely, this correlation could not be shown in the MTT assays. The MTT results, which are based on the mitochondrial activity, were confirmed by analysis of the succinate dehydrogenase complex by immunofluorescence and by western blotting. Taken together, our study shows that Wnt3a activates proliferation of HEK293 cells. These effects can be detected by measuring DNA synthesis rather than by measuring changes of mitochondrial activity.

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Metadaten
Author:Patricia Reischmann, Johanna FiebeckORCiDGND, Nadine von der Weiden, Oliver Müller
URN:urn:nbn:de:bsz:960-opus4-30784
DOI:https://doi.org/10.25968/opus-3078
DOI original:https://doi.org/10.1155/2015/928502
ISSN:1687-8884
Parent Title (English):International Journal of Cell Biology
Document Type:Article
Language:English
Year of Completion:2015
Publishing Institution:Hochschule Hannover
Release Date:2024/04/09
GND Keyword:Wnt-Proteine
Article Number:928502
Page Number:8
Institutes:Fakultät III - Medien, Information und Design
DDC classes:610 Medizin, Gesundheit
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International