TY - JOUR U1 - Wissenschaftlicher Artikel A1 - Woudstra, Svenja A1 - Wente, Nicole A1 - Zhang, Yanchao A1 - Leimbach, Stefanie A1 - Kirkeby, Carsten A1 - Gussmann, Maya Katrin A1 - Krömker, Volker T1 - Reservoirs of Staphylococcus spp. and Streptococcus spp. Associated with Intramammary Infections of Dairy Cows JF - Pathogens N2 - To design cost-effective prevention strategies against mastitis in dairy cow farms, knowledge about infection pathways of causative pathogens is necessary. Therefore, we investigated the reservoirs of bacterial strains causing intramammary infections in one dairy cow herd. Quarter foremilk samples (n = 8056) and milking- and housing-related samples (n = 251; from drinking troughs, bedding material, walking areas, cow brushes, fly traps, milking liners, and milker gloves), were collected and examined using culture-based methods. Species were identified with MALDI-TOF MS, and selected Staphylococcus and Streptococcus spp. typed with randomly amplified polymorphic DNA-PCR. Staphylococci were isolated from all and streptococci from most investigated locations. However, only for Staphylococcus aureus, matching strain types (n = 2) were isolated from milk and milking-related samples (milking liners and milker gloves). Staphylococcus epidermidis and Staphylococcus haemolyticus showed a large genetic diversity without any matches of strain types from milk and other samples. Streptococcus uberis was the only Streptococcus spp. isolated from milk and milking- or housing-related samples. However, no matching strains were found. This study underlines the importance of measures preventing the spread of Staphylococcus aureus between quarters during milking. KW - mastitis prevention KW - staphylococci KW - streptococci KW - pathogen reservoirs KW - environmental reservoirs KW - transmission pathways KW - Euterentzündung KW - Prävention KW - Staphylococcus KW - Streptococcus KW - Pathogener Mikroorganismus KW - Krankheitsübertragung Y1 - 2023 UN - https://nbn-resolving.org/urn:nbn:de:bsz:960-opus4-28759 SN - 2076-0817 SS - 2076-0817 U6 - https://doi.org/10.25968/opus-2875 DO - https://doi.org/10.25968/opus-2875 N1 - Gefördert mit Mitteln des Publikationsfonds der Bibliothek der Hochschule Hannover. VL - 12 IS - 5 ER -