TY  - JOUR
U1  - Wissenschaftlicher Artikel
A1  - Lücken, Anneke
A1  - Wente, Nicole
A1  - Zhang, Yanchao
A1  - Woudstra, Svenja
A1  - Krömker, Volker
T1  - Corynebacteria in Bovine Quarter Milk Samples—Species and Somatic Cell Counts
JF  - Pathogens
N2  - In this species differentiation study of Corynebacterium spp. (C. spp.), quarter foremilk samples from 48 farms were included. These were obtained from both clinically healthy cows and those with clinical mastitis. First, all samples were examined cyto-microbiologically and all catalase-positive rods were differentiated using the direct transfer method in MALDI-TOF MS. C. bovis, C. amycolatum, C. xerosis, and five other species were identified with proportions of 90.1%, 7.7%, and 0.8% for the named species, respectively, and 1.4% for the remaining unnamed species. In addition, somatic cell count (SCC) was determined by flow cytometry. Based on this, the isolates were classified into four udder health groups: “latent infection”, “subclinical mastitis”, “clinical mastitis” and “others”. Approximately 90% of isolates of C. bovis and C. amycolatum were from latently and subclinically infected quarters. Of the C. bovis isolates, 5.8% were obtained from milk samples from clinical mastitis, whereas C. amycolatum was not present in clinical mastitis. The distribution of groups in these two species differed significantly. The geometric mean SCC of all species combined was 76,000 SCC/mL, almost the same as the SCC of C. bovis. With 50,000 SCC/mL, the SCC of C. amycolatum was slightly below the SCC of C. bovis. Through the species-level detection and consideration of SCC performed here, it is apparent that individual species differ in terms of their pathogenicity. Overall, their classification as minor pathogens with an SCC increase is confirmed.
KW  - bovine mastitis
KW  - Corynebacteria
KW  - SCC
KW  - MALDI-TOF MS
KW  - species diagnostics
KW  - Euterentzündung
KW  - Milchvieh
KW  - Corynebacterium
KW  - MALDI-MS
KW  - Somazelle
Y1  - 2021
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:960-opus4-19682
SN  - 2076-0817
SS  - 2076-0817
U6  - https://doi.org/10.25968/opus-1968
DO  - https://doi.org/10.25968/opus-1968
N1  - Gefördert mit Mitteln des Publikationsfonds der Bibliothek der Hochschule Hannover.
VL  - 10
IS  - 7
ER  -