TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Speksnijder, David C.
A1  - Hopman, Nonke E. M.
A1  - Kusters, Nina E.
A1  - Timmerman, Arjen
A1  - Swinkels, Jantijn M.
A1  - Penterman, Pleun A. A.
A1  - Krömker, Volker
A1  - Bradley, Andrew J.
A1  - Botteldoorn, Nadine
A1  - Gehring, Ronette
A1  - Zomer, Aldert L.
T1  - Potential of ESBL-producing Escherichia coli selection in bovine feces after intramammary administration of first generation cephalosporins using in vitro experiments
JF  - Scientific Reports
N2  - Selection and spread of Extended Spectrum Beta-Lactamase (ESBL) -producing Enterobacteriaceae within animal production systems and potential spillover to humans is a major concern. Intramammary treatment of dairy cows with first-generation cephalosporins is a common practice and potentially selects for ESBL-producing Enterobacteriaceae, although it is unknown whether this really occurs in the bovine fecal environment. We aimed to study the potential effects of intramammary application of cephapirin (CP) and cefalonium (CL) to select for ESBL-producing Escherichia coli in the intestinal content of treated dairy cows and in manure slurry, using in vitro competition experiments with ESBL and non-ESBL E. coli isolates. No selection of ESBL-producing E. coli was observed at or below concentrations of 0.8 µg/ml and 4.0 µg/ml in bovine feces for CP and CL, respectively, and at or below 8.0 µg/ml and 4.0 µg/ml, respectively, in manure slurry. We calculated that the maximum concentration of CP and CL after intramammary treatment with commercial products will not exceed 0.29 µg/ml in feces and 0.03 µg/ml in manure slurry. Therefore, the results of this study did not find evidence supporting the selection of ESBL-producing E. coli in bovine feces or in manure slurry after intramammary use of commercial CP or CL-containing products.
KW  - Antibiotics
KW  - Antimicrobial resistance
KW  - Bioinformatics
KW  - Pathogens
KW  - Public health
KW  - Antibiotikum
KW  - Bioinformatik
KW  - Extended-Spectrum Beta-Lactamase
KW  - Rind
KW  - Gülle
KW  - Escherichia coli
KW  - Pathogene Bakterien
Y1  - 2022
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:960-opus4-32003
SN  - 2045-2322
SS  - 2045-2322
U6  - https://doi.org/10.25968/opus-3200
DO  - https://doi.org/10.25968/opus-3200
VL  - 12
SP  - 10
S1  - 10
PB  - Springer Nature
ER  -