TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Setyawati, Andina
A1  - Wahyuningsih, Mae Sri Hartati
A1  - Nugrahaningsih, Dwi Aris Agung
A1  - Effendy, Christantie
A1  - Fneish, Firas
A1  - Fortwengel, Gerhard
T1  - Piper crocatum Ruiz & Pav. ameliorates wound healing through p53,E-cadherin and SOD1 pathways on wounded hyperglycemia fibroblasts
JF  - Saudi Journal of Biological Sciences
N2  - Introduction: Piper crocatum Ruiz & Pav (P. crocatum) has been reported to accelerate the diabetic wound healing process empirically. Some studies showed the benefits of P. crocatum in treating various diseases but its mechanisms in diabetic wound healing have never been reported. In the present study we investigated the diabetic wound healing activity of the active fraction of P. crocatum on wounded hyperglycemia fibroblasts (wHFs).
Methods: Bioassay-guided fractionation was performed to get the most active fraction. The selected active fraction was applied to wHFs within 72 h incubation. Mimicking a diabetic condition was done using basal glucose media containing an additional 17 mMol/L D-glucose. A wound was simulated via the scratch assay. The collagen deposition was measured using Picro-Sirius Red and wound closure was measured using scratch wound assay. Underlying mechanisms through p53, aSMA, SOD1 and Ecadherin were measured using western blotting.
Results: We reported that FIV is the most active fraction of P. crocatum. We confirmed that FIV\(7.81 mg/ml, 15.62 mg/ml, 31.25 mg/ml, 62.5 mg/ml, and 125 mg/ml) induced the collagen deposition and wound closure of wHFs. Furthermore, FIV treatment (7.81 mg/ml, 15.62 mg/ml, 31.25 mg/ml) down-regulated the protein expression level of p53 and up-regulated the protein expression levels of aSMA, E-cadherin, and SOD1.
Discussion/conclusions: Our findings suggest that ameliorating collagen deposition and wound closure through protein regulation of p53, aSMA, E-cadherin, and SOD1 are some of the mechanisms by which FIV of P. crocatum is involved in diabetic wound healing therapy.
KW  - Piper crocatum Ruiz & Pav
KW  - Diabetic wound healing
KW  - Mechanism
KW  - p53
KW  - E-cadherin
KW  - SOD1
KW  - Diabetes mellitus
KW  - Wundheilung
KW  - Fraktionierung
Y1  - 2021
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:960-opus4-21148
SN  - 1319-562X
SS  - 1319-562X
U6  - https://doi.org/10.25968/opus-2114
DO  - https://doi.org/10.25968/opus-2114
VL  - 28
IS  - 12
SP  - 7257
EP  - 7268
ER  -